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Immobilization of microbial cells containing NAD‐kinase
Author(s) -
Hayashi Toru,
Tanaka Yoshikazu,
Kawashima Koji
Publication year - 1979
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260210607
Subject(s) - nad+ kinase , chemistry , brevibacterium , biochemistry , bacteria , enzyme , microbiology and biotechnology , microorganism , biology , genetics
Abstract Microbial cells having NAD‐kinase activity, Brevibacterium ammoniagenes , were immobilized by the radiation‐copolymerization method under low temperature with the activity recovery of more than 80%. Compared to the native microbial cells the immobilized cells were more stable against heat and pH change. The immobilized cells were subjected to the 5 hr reaction repeatedly 20 times without any activity loss.