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Enzyme immobilization by radiation‐induced polymerization of hydrophobic glass‐forming monomers at low temperatures
Author(s) -
Kaetsu Isao,
Kumakura Minoru,
Yoshida Masaru
Publication year - 1979
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260210509
Subject(s) - monomer , polymerization , copolymer , yield (engineering) , polymer chemistry , chemistry , chemical engineering , particle (ecology) , immobilized enzyme , hydrophobic effect , materials science , organic chemistry , enzyme , polymer , composite material , oceanography , engineering , geology
Enzyme immobilization was studied by means of radiation‐induced polymerization of hydrophobic glass‐forming monomers at low temperatures. The polymerized hydrophobic composite was generally obtained in microspheric form. Enzymatic activity showed little decrease with repeated use in these systems. The particle size of the microsphere increased with increasing monomer concentration, and activity yield had a maximum at an optimum monomer concentration. Immobilization by copolymerization of hydrophilic and hydrophobic comonomers was also investigated and a maximum activity yield was found at a certain monomer concentration. A model scheme for immobilization at low temperatures was proposed and discussed.