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Large‐scale isolation of equine liver alcohol dehydrogenase on a blue agarose gel
Author(s) -
Roy S. K.,
Nishikawa A. H.
Publication year - 1979
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260210504
Subject(s) - agarose , alcohol dehydrogenase , sepharose , chromatography , enzyme , chemistry , adsorption , affinity chromatography , biochemistry , isolation (microbiology) , sorbent , biology , microbiology and biotechnology , organic chemistry
Equine liver alcohol dehydrogenase (EC 1.1.1.1) has been purified by a new scheme using a blue agarose gel (Blue Sepharose) as an affinity sorbent. Starting amounts of 0.6 to 10 kg liver have been processed to enzyme possessing 1.5 U/mg average specific activity, in about three to four days. Some parameters concerning adsorption of enzyme to the blue gel as well as recovery therefrom have been explored.