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Bioprobe electrode for phenol
Author(s) -
Neujahr Halina Y.,
Kjellén Karin G.
Publication year - 1979
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260210411
Subject(s) - phenol , electrode , chromatography , clark electrode , chemistry , fermentation , agar , yeast , sewage , thin layer , layer (electronics) , biochemistry , biology , organic chemistry , environmental science , bacteria , environmental engineering , electrolyte , genetics
A clark oxygen electrode is coated with a thin layer of cell paste of induced trichosporon cutaneum . This bioprode enables one to perform rapid, quantitative determination of phenol in the 0–15 mg/liter range. The assay uses only 2–5 ml and is complete 15 sec after adding the sample. The bioprobe is stable for at least five days at room temperature. It lasts at least 100 assays. Induced yeast cells come from shake cultures or agar plates. They can be freeze‐stored for several weeks before mounting on the electrode. A method of estimating corrections for other exidizable substances is exemplified. The bioprobe can be used to measure phenol in water, fermentation broth, and municipal sewage.