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Process characteristics of cell lysis mutants of Saccharomyces cerevisisae
Author(s) -
Boudrant Joseph,
DeAngelo Joseph,
Sinskey Anthony J.,
Tannenbaum Steven R.
Publication year - 1979
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260210410
Subject(s) - lysis , saccharomyces cerevisiae , mutant , alkaline phosphatase , yeast , biochemistry , cell , phosphatase , chemistry , microbiology and biotechnology , cell growth , enzyme , biology , gene
Several temperature‐sensitive lysis mutants of Saccharomyces cerevisiae were selected according to their ability to release alkaline phosphatase when incubated at a nonpermissive temperature. For two mutants, cell lysis and release of alkaline phosphatase reached a maximum when cells in the logarithmic growth phase were shifted to the ncnpermissive temperature. Morphological changes, as well as changes in macromolecular composition of the cells, were observed. Growth is necessary and oxygen is important for the expression of cell lysis at the nonpermisseve temperature.