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Immobilization of enzymes on activated carbon: Selection and preparation of the carbon support
Author(s) -
Cho Y. K.,
Bailey J. E.
Publication year - 1979
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260210308
Subject(s) - chemistry , activated carbon , catalysis , immobilized enzyme , glutaraldehyde , amination , silanization , carbon fibers , adsorption , organic chemistry , nuclear chemistry , enzyme , materials science , composite number , composite material
Based upon its superior catalytic activity for H 2 O 2 decomposition, a bituminous coal‐based activated carbon was selected for investigations of pretreatment and enzyme immobilization methods. Pretreatments considered include acid washing, exposure to strong oxidizing agents, contact with concentrated peroxide solutions, nitration and amination, isothiocyanate derivatization, silanization, and stearic acid coating. Effects of these pretreatments on morphology and trace‐metal content of the carbon pellets have been studied using scanning electron microscopy and dispersive analysis of x rays. Immobilization of glucoamylase by adsorption, glutaraldehyde crosslinking, and covalent attachment to carbon activated by water‐soluble diimide or diazotization have been examined. These different enzyme–carbon catalysts have been characterized by their enzyme loading, enzyme activity, catalytic activity for H 2 O 2 decomposition, or combinations of these measures of performance.