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Human interferon production with diploid fibroblast cells grown on microcarriers
Author(s) -
Giard Donald J.,
Loeb David H.,
Thilly W. G.,
Wang D. I. C.,
Levine D. W.
Publication year - 1979
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260210306
Subject(s) - microcarrier , ploidy , fibroblast , interferon , wi 38 , microbiology and biotechnology , biology , cell culture , chemistry , virology , genetics , gene
A variety of diploid human fibroblast lines have been successfully grown to high densities (>10 6 cell/ml) on recently developed microcarriers. Interferon induction using poly I·poly C and a superinduction procedure resulted in yields greater than 10,000 units/ml with one cell line. A direct comparison of microcarrier cultures to roller bottle cultures showed equivalent interferon yields on a per cell basis and some apparent differences relating to optimum inducer concentrations and kinetics of interferon accumulation.