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Production of citric acid in continuous culture
Author(s) -
Kristiansen B.,
Sinclair C. G.
Publication year - 1979
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260210214
Subject(s) - citric acid , dilution , nitrogen , chemistry , limiting , food science , biochemistry , chromatography , organic chemistry , engineering , mechanical engineering , physics , thermodynamics
Work has been carried out on the production of citric acid by Aspergillus foetidus in single‐stage continuous culture, operated under nitrogen‐limiting conditions at dilution rates between 0.04 to 0.21 hr −1 . Citric acid concentration increased rapidly as the dilution rate decreased and appears to be critically dependent on the pH in the culture vessel and the nitrogen concentration in the feed. A mathematical model based on a distinction between basic cells, which require nitrogen but do not produce citric acid, and stroage cells, which accumulate carbon and simultaneously produce citric acid, is proposed.