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Penicillin G production by immobilized whole cells of Penicillium chrysogenum
Author(s) -
Morikawa Yasushi,
Karube Isao,
Suzuki Shuichi
Publication year - 1979
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260210211
Subject(s) - penicillium chrysogenum , mycelium , penicillin , acrylamide , chemistry , chromatography , food science , biochemistry , monomer , biology , antibiotics , botany , organic chemistry , polymer
Abstract Penicillium chrysogenum was immobilized in polyacrylamide gel prepared from 5% acrylamide monomers (85% acrylamide and 15% N , N ′‐methylene bisacrylamide). Penicillin produced from glucose by the immobilized mycelium was 17% of that produced by washed mycelium. However, the activity of penicillin production of the washed mycelium decreased with repeated use. On the other hand, the activity of the immobilized mycelium increased initially and decreased gradually with repeated use. The rate of oxygen uptake of the immobilized mycelium was about 30% of that of the washed mycelium. The immobilized mycelium required oxygen for the production of penicillin.