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Disintegration of dried yeast cells and its effect on protein extractability, sedimentation property, and viscosity of the cell suspension
Author(s) -
Lee CherlHo,
Tsang Shek Kwan,
Urakabe Rintaro,
Rha Cho Kyun
Publication year - 1979
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260210102
Subject(s) - homogenization (climate) , aqueous solution , acetone , cell wall , solubility , scanning electron microscope , chemistry , yeast , chromatography , suspension (topology) , chemical engineering , nuclear chemistry , materials science , biochemistry , organic chemistry , composite material , biology , biodiversity , ecology , mathematics , homotopy , pure mathematics , engineering
The morphology of dried Candida lipolytica yeast suspended in aqueous solutions (H 2 O, 0.4% NaOH, 2 N HCl, and 6 N HCl) and organic solvents (95% alcohol and acetone) was studied using a scanning electron microscope (SEM) and an optical microscope. The effect of high‐pressure homogenization on cell‐wall structure and cell clumps was also determined. The protein extractability, sedimentation property, and viscosity of cells subjected to different mechanical and chemical treatments were also investigaged. The dried yeast cells were in a spherical agglomeration consisting of 100s of closely bound cells. The clump was resistant to water, aqueous 2 N HCl solution at 25°C, 95% alcohol and acetone, but vulnerable to 6 N HCl, aqueous 0.4% NaOH solution, and homogenization. The homogenization of the cell suspension not only broke the clump but also cracked the cell‐wall structure. The aqueous alkaline solution could have weakened the cell wall and increased the solubility of the protein released through the cracks in the cell wall. The destruction of the agglomeration and the cell‐wall structure increased the hydration of the cell and thereby increased the stability of the suspension. The sedimentation and the viscosity of the cell suspension corresponded to the morphological changes and the extractability of protein in the cell suspensions with different treatments.