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Continuous culture of Candida lipolytica on n ‐hexadecane
Author(s) -
Gutierrez J. R.,
Erickson L. E.
Publication year - 1978
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260201111
Subject(s) - hexadecane , hydrocarbon , sauter mean diameter , surface tension , volume fraction , fraction (chemistry) , chemistry , chemical engineering , volumetric flow rate , chromatography , steady state (chemistry) , kinetics , materials science , thermodynamics , organic chemistry , physics , nozzle , engineering , quantum mechanics
Candida lipolytica was grown continuously on n ‐hexadecane as the main source of carbon. A transient continuous‐culture experiment was also conducted to investigate hydrocarbon‐limited growth; the hydrocarbon feed flow rate was stopped for several hours and then resumed at a reduced steady‐state flow rate. Interfacial tension, Sauter mean diameter, pseudosolubility, fraction of cells in the aqueous phase, oil‐phase volume fraction, and cell concentration were measured to characterize the system. The microorganisms appear to utilize both the submicron drops and the microscopic drops. The effects of interfacial tension, pseudosolubility, and unoccupied interfacial area on the kinetics of hydrocarbon fermentation are discussed here. A conceptual model for hydrocarbon uptake is presented and discussed.