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Model for oxygen transfer in a shake flask
Author(s) -
Van Suijdam J. C.,
Kossen N. W. F.,
Joha A. C.
Publication year - 1978
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260201102
Subject(s) - aeration , limiting , shake , oxygen , mass transfer , mass transfer coefficient , chemistry , environmental science , chromatography , mechanical engineering , engineering , organic chemistry
The shake flask is a very common and useful tool for the study of submerged fermentations on a small scale. However, the oxygen supply may easily become a limiting factor. A model for the aeration in shake flasks is presented that enables one to predict whether in the course of an experiment the oxygen supply is becoming a growth‐limiting factor or not. The results of measurements of the transfer coefficient of a cotton plug and the oxygen mass‐transfer coefficient k l a are also given.