Premium
Immobilization of amyloglucosidase on poly [(glycidyl methacrylate) Co(ethylene dimethacrylate)] carrier and its derivatives
Author(s) -
Švec F.,
Kálal J.,
Menyailova I. I.,
Nakhapetyan L. A.
Publication year - 1978
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260200902
Subject(s) - glutaraldehyde , chemistry , glycidyl methacrylate , starch , aldehyde , nuclear chemistry , amylase , ethylene , methacrylate , aspergillus niger , epoxide , aspergillus oryzae , polymer chemistry , immobilized enzyme , enzyme , copolymer , organic chemistry , biochemistry , catalysis , polymer
Immobilization of amyloglucosidase (EC 3.2.1.3) from Endomycopsis bispora and Aspergillus niger was followed with carriers containing epoxide, aldehyde, and primary amino groups. Determinations of stability of bound enzyme showed that the most active and stable preparations were obtained by application of the carrier with amino groups activated with glutaraldehyde (activity half‐life 96.6 days). Optimum pH and and temperature were found for cleavage of starch solution and K M (app) and V max (app) values were determined for all prepared samples.