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Continuous fractionation of human plasma
Author(s) -
Mitra Gautam,
Lundblad John
Publication year - 1978
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260200706
Subject(s) - fractionation , chemistry , chromatography , human plasma , protein precipitation , precipitation , continuous flow , yield (engineering) , plasma , ethanol , biochemistry , materials science , physics , quantum mechanics , meteorology , mechanics , metallurgy
Continuous processing has been applied to human plasma fractionation by the cold ethanol process. On‐line pH control of ±0.05 pH units, flow control of ±1%, and temperature control of ±0.5°C have been achieved. Optimization of precipitation pHs has been carried out for purifying plasma protein fractions and albumin. During precipitation, the irreversible nature of the pH overshoots has been demonstrated. Compared to the batch processing mode, the continuous scheme produces an increased yield between 6 to 11%.