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Automatic assay of cellulase activity during fermentation
Author(s) -
Leisola Matti,
Kauppinen Veli
Publication year - 1978
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260200606
Subject(s) - cellulase , trichoderma viride , fermentation , cellulose , chemistry , substrate (aquarium) , enzyme , enzyme assay , chromatography , biochemistry , food science , biology , ecology
An automatic method using dyed cellulose as a substrate was developed to determine the solubilizing activity of a cellulase complex. The automatic analyzer was connected to a fermentor and enzyme activity was successfully assayed during fermentation. A useful sampling arrangement was developed and the analysis was sensitive enough to make a short reaction time possible. Cellulase production with Trichoderma viride was used as a model process.