Automatic assay of cellulase activity during fermentation | Zendy

Leisola Matti | Zendy; Kauppinen Veli | Zendy

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Automatic assay of cellulase activity during fermentation

Author(s) -

Leisola Matti,

Kauppinen Veli

Publication year - 1978

Publication title -

biotechnology and bioengineering

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.136

H-Index - 189

eISSN - 1097-0290

pISSN - 0006-3592

DOI - 10.1002/bit.260200606

Subject(s) - cellulase , trichoderma viride , fermentation , cellulose , chemistry , substrate (aquarium) , enzyme , enzyme assay , chromatography , biochemistry , food science , biology , ecology

An automatic method using dyed cellulose as a substrate was developed to determine the solubilizing activity of a cellulase complex. The automatic analyzer was connected to a fermentor and enzyme activity was successfully assayed during fermentation. A useful sampling arrangement was developed and the analysis was sensitive enough to make a short reaction time possible. Cellulase production with Trichoderma viride was used as a model process.

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