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Immobilization of Streptomyces flavochromogenes pullulanase on tannic acid and TEAE–cellulose
Author(s) -
Ohba Riichiro,
Chaen Hiroto,
Hayashi Sachio,
Ueda Seinosuke
Publication year - 1978
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260200504
Subject(s) - pullulanase , tannic acid , cellulose , chemistry , streptomyces , biochemistry , microbiology and biotechnology , organic chemistry , bacteria , enzyme , biology , genetics
Pullulanase was immobilized successfully by simple, inexpensive methods that may be useful for industrial application of this enzyme. A tannin–pullulanase(TP) complex was obtained by addition of tannic acid to the culture filtrate of thermophilic Streptomyces flavochromogenes . TP could be bound to TEAE–cellulose (TTCP). Immobilization in this manner took place with quantitative retention of activity. The immobilized enzymes were stable for more than six months. The optimum temperatures of the native enzyme and TP were both 50°C; that of TTCP was 45°C. In the presence of 5m M Ca 2+ , the activity of TTCP was increased approximately twofold and the optimum temperature was raised to 50–60°C. Pullulanase was not significantly eluted from TP or TTCP by NaCl Solution (0.1–0.5 M ).