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Kinetics of enzymatic hydrolysis of cellulose: Analytical description of a mechanistic model
Author(s) -
Okazaki M.,
MooYoung M.
Publication year - 1978
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260200503
Subject(s) - cellulose , kinetics , chemistry , enzymatic hydrolysis , hydrolysis , substrate (aquarium) , enzyme , beta (programming language) , degree of polymerization , michaelis–menten kinetics , glucanase , organic chemistry , polymerization , stereochemistry , chromatography , enzyme assay , computer science , biology , physics , ecology , quantum mechanics , programming language , polymer
A generalized mechanistic model for the enzymatic hydrolysis of cellulose is developed and expressed mathematically. The model is based on Michaelis–Menten‐type kinetics for concurrent random and endwise attack of the substrate involving end‐product inhibitions and three types of enzymes: an endo‐β;‐1,4‐glucanase, an exo‐β‐1,4‐glucanase, and β‐glucosidase. Basic parameters of the model which can explain synergistic and other effects observed experimentally are quantified and discussed. It is shown that cellulose degradation kinetics are expected to be strongly affected by the ratio of endo‐ to exocellulases in the reaction mixture as indicated by previous experimental data, and the substrate degree of polymerization, a factor not fully appreciated in previous studies, which appear to be overridingly important in many practical cases.