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Urocanic acid production using whole cells immobilized in a hollow fiber reactor
Author(s) -
Kan J. K.,
Shuler M. L.
Publication year - 1978
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260200205
Subject(s) - urocanic acid , chemistry , pseudomonas fluorescens , histidine , membrane , ammonia , fiber , chromatography , biochemistry , chemical engineering , nuclear chemistry , organic chemistry , enzyme , bacteria , biology , engineering , genetics
The feasibility of using hollow fiber membrane dialyzers (C‐DAK) for immobilization of microbial whole cells was investigated. The cells are located on the shell side of the dialyzer, while substrates and products are free to diffuse across the hollow fiber membranes. The biochemical reaction studied was the conversion of L ‐histidine to urocanic acid and catalyzed by L ‐histidine ammonia‐lyase. C‐DAK dialyzers containing a heat‐treated suspension of Pseudomonas fluorescens ATCC 11299b (with L ‐histidine ammonia–lyase activity) were incorporated into constant volume recycle reactor systems for continuous product formation. A simple model successfully correlated the data and predicted performance. It was found that the reaction was not likely to be diffusion limited, and such a cell immobilization scheme is convenient and workable for continuous production of biochemicals.