Investigation of the unit operations involved in the continuous flow isolation of β‐galactosidase from  Escherichia coli | Zendy

Higgins J. J. | Zendy; Lewis D. J. | Zendy; Daly W. H. | Zendy; Mosqueira F. G. | Zendy; Dunnill P. | Zendy; Lilly M. D. | Zendy

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Investigation of the unit operations involved in the continuous flow isolation of β‐galactosidase from Escherichia coli

Author(s) -

Higgins J. J.,

Lewis D. J.,

Daly W. H.,

Mosqueira F. G.,

Dunnill P.,

Lilly M. D.

Publication year - 1978

Publication title -

biotechnology and bioengineering

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.136

H-Index - 189

eISSN - 1097-0290

pISSN - 0006-3592

DOI - 10.1002/bit.260200202

Subject(s) - escherichia coli , fermentation , nucleic acid , chromatography , isolation (microbiology) , chemistry , yield (engineering) , continuous flow , biochemistry , biology , microbiology and biotechnology , materials science , biochemical engineering , gene , metallurgy , engineering

A 1000 liter fermentor has been used to produce a continuous feed of Escherichia coli containing a high level of β‐galactosidase. We have investigated the individual unit operations for the isolation of the enzyme: cell disruption, nucleic acid removal, protein precipitation, and solid–liquid separation after each stage. Using the information obtained we have been able to operate a semicontinuous process which when fully continuous would yield 100 g protein/hr, comprising 23% β‐galactosidase.

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