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Investigation of the unit operations involved in the continuous flow isolation of β‐galactosidase from Escherichia coli
Author(s) -
Higgins J. J.,
Lewis D. J.,
Daly W. H.,
Mosqueira F. G.,
Dunnill P.,
Lilly M. D.
Publication year - 1978
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260200202
Subject(s) - escherichia coli , fermentation , nucleic acid , chromatography , isolation (microbiology) , chemistry , yield (engineering) , continuous flow , biochemistry , biology , microbiology and biotechnology , materials science , biochemical engineering , gene , metallurgy , engineering
A 1000 liter fermentor has been used to produce a continuous feed of Escherichia coli containing a high level of β‐galactosidase. We have investigated the individual unit operations for the isolation of the enzyme: cell disruption, nucleic acid removal, protein precipitation, and solid–liquid separation after each stage. Using the information obtained we have been able to operate a semicontinuous process which when fully continuous would yield 100 g protein/hr, comprising 23% β‐galactosidase.