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Synthesis of a soluble catalase–dextran conjugate
Author(s) -
Marshall J. John,
Humphreys John D.
Publication year - 1977
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260191202
Subject(s) - conjugate , cyanogen bromide , dextran , catalase , chemistry , bromide , combinatorial chemistry , enzyme , chromatography , biochemistry , organic chemistry , peptide sequence , mathematical analysis , mathematics , gene
Results of a systematic study of the conditions for preparation of soluble catalase–dextran conjugates, using the cyanogen bromide activation procedure, are reported. A protocol for the synthesis of such a conjugate with satisfactory retention of enzymatic activity and high efficiency of coupling is described.