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Media suitable for industrial production of Entomophthora virulenta zygospores
Author(s) -
Latgé J.P.,
Soper R. S.,
Madore C. D.
Publication year - 1977
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260190903
Subject(s) - spore , erlenmeyer flask , nutrient , laboratory flask , germination , biology , botany , food science , chemistry , chromatography , ecology
Entomophthora virulenta has been grown using inexpensive, commercial grade nutrient sources in order to produce a resting spore formulation available to agriculture. Solid media and liquid media in shake flasks and fermentors were examined. The best carbon sources for sporulation were dextrose and corn syrup, while the best nitrogen sources were yeast extract, soybean, and cottonseed flours. The optimal nutrient concentration (12%) produced 3 × 10 6 spores/ml with a 70% germination rate within five days.