Premium
Hydrolysis of cellulose in a cellulase‐bead fluidized bed reactor
Author(s) -
Karube Isao,
Tanaka Shigeru,
Shirai Takashi,
Suzuki Shuichi
Publication year - 1977
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260190808
Subject(s) - cellulase , cellulose , fluidized bed , hydrolysis , chemistry , chromatography , chemical engineering , materials science , organic chemistry , engineering
Cellulase was immobilized in a collagen fibril matrix, and no leakage of cellulase from the collagen fibril matrix was observed. The immobilized cellulase was more stable than the native cellulase. The substrate cellulose was hydrolyzed quantitatively with immobilized cellulase. The final reaction product was identified as glucose. Immobilized cellulase was used in a fluidized bed reactor where the pressure drop of the fluidized bed reactor was low and constant. Cellulose was hydrolyzed to glucose by the cellulase‐bead fluidized bed reactor. The minimum flow velocity ( U mf ) was 0.5 cm/sec and the optimum flow velocity of the cellulose hydrolysis was 1 cm/sec.