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Corynecin (chloramphenicol analogs) fermentation studies: Selective production of Corynecin I by Corynebacterium hydrocarboclastus grown on acetate
Author(s) -
Nakano Hirofumi,
Tomita Fusao,
Yamaguchi Ken,
Nagashima Minoru,
Suzuki Takeo
Publication year - 1977
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260190705
Subject(s) - fermentation , yield (engineering) , chemistry , chloramphenicol , phosphate , ammonium acetate , ammonium , ethyl acetate , food science , chromatography , nuclear chemistry , organic chemistry , biochemistry , antibiotics , materials science , high performance liquid chromatography , metallurgy
Corynebacterium hydrocarboclastus KY 8835 grew in the acetate medium and accumulated 28m M Corynecins which was the highest production yield among the processes using various carbon sources. Selective production of Corynecin I (over 90% of all Corynecins), which had been desired for increase of the product yield, was achieved in this acetate medium. To keep the concentration of acetate, ammonium, and phosphate ions in the optimum range throughout the fermentation, a solution containing CH 3 COOH (50%), CH 3 COONH 4 (9%), and KH 2 PO 4 (0.2%) was fed continuously to the culture medium as the pH controlling agent. The addition of KCl (1%) and NaCl (1%) to the medium at 12 hr after inoculation stimulated the production of Corynecins.