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Growth kinetics and cellulase biosynthesis in the continuous culture of Trichoderma viride
Author(s) -
Brown D. E.,
Zainudeen M. A.
Publication year - 1977
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260190702
Subject(s) - cellulase , trichoderma viride , dilution , chemistry , fermentation , oxygen , biochemistry , steady state (chemistry) , chromatography , botany , food science , enzyme , biology , thermodynamics , organic chemistry , physics
Continuous culture studies have been carried out growing Trichoderma viride QM 9123 in a 10 liter stirred fermentor on a medium containing commercial glucose as the carbon source. Experiments were carried out at 30°C and at three controlled pH values of 2.5, 3.0, and 4.0, over a range of dilution rates from 0.01 to 0.11 hr −1 . Steady‐state values of cell, glucose, and cellulase concentration oxygen tension, and outlet gas oxygen partial pressure were recorded. Values of maximum specific growth rate, endogenous metabolism coefficient, Michaelis‐Menten coefficient, yield and maintenance coefficient for glucose were derived and correlated the effect of the hydrogen ion concentration. Specific oxygen uptake rates were correlated with specific growth rates and absorption coefficients were shown to be a function of dilution rate independent of pH. Some data on cellulase biosynthesis were examined and correlated in terms of a maturation time model.