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The immobilization of microbial cells, subcellular organelles, and enzymes in calcium alginate gels
Author(s) -
Kierstan M.,
Bucke C.
Publication year - 1977
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260190309
Subject(s) - calcium alginate , chemistry , calcium , biochemistry , glucose oxidase , kluyveromyces marxianus , enzyme , immobilized enzyme , chloroplast , mitochondrion , glucuronic acid , saccharomyces cerevisiae , yeast , polysaccharide , organic chemistry , gene
Saccharomyces cerevisiae cells, Kluyveromyces marxianus cells, inulase, glucose oxidase, chloroplasts, and mitochondria were immobilized in calcium alginate gels. Ethanol production from glucose solutions by an immobilized preparation of S. cerevisiae was demonstrated over a total of twenty‐three days, and the half‐life of such a preparation was shown to be about ten days. Immobilized K. marxianus , inulase, and glucose oxidase preparations were used to demonstrate the porosity and retraining properties of calcium alginate gels. Calcium alginate–immobilized chloroplasts were shown to perform the Hill reaction. Some experiments with immobilized mitochondria are reported.