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Enzymatic treatment of primary municipal sludge with Trichoderma viride cellulase
Author(s) -
CinqMars Gerald V.,
Howell John
Publication year - 1977
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260190308
Subject(s) - cellulase , trichoderma viride , cellulose , chemistry , pulp and paper industry , cellobiose , slurry , enzymatic hydrolysis , raw material , hydrolysis , filter paper , cellulosic ethanol , food science , waste management , chromatography , biochemistry , organic chemistry , environmental science , environmental engineering , engineering
Enzymatic hydrolysis of the cellulose in raw primary settled municipal sludge by Trichoderma viride cellulase achieved conversions of up to 75% of the cellulose, primarily to cellobiose, in 24 hr. Simultaneously the gel‐like characteristic of raw primary sludge was changed to that of a slurry of fine particles in less than 2 hr, causing a radical change in the ability to ultrafilter the sludge. The use of raw primary sludge as a growth medium for T. viride cellulase production was also investigated. It was possible, with nitrogen supplements, to obtain an enzyme with a filter paper activity (FPA) of two compared to over four which is attainable on defined medium of similar strength. The potential use of cellulase treatment as a pretreatment or integral part of waste treatment processes is discussed and the alternatives evaluated.