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Bacteriolysis by immobilized enzymes
Author(s) -
Karube Isao,
Suganuma Toshiro,
Suzuki Shuichi
Publication year - 1977
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260190302
Subject(s) - enzyme , xanthomonas oryzae , microbiology and biotechnology , biochemistry , bacteria , pseudomonas aeruginosa , pseudomonas , chemistry , ralstonia solanacearum , staphylococcus aureus , xanthomonas , membrane , biology , pathogen , genetics , gene
Bacteriolytic enzymes produced by Achromobacter lunatus were immobilized in collagen membrane. Intact bacteria such as Pseudomonas solanacearum, Xanthomonas oryzae, Staphylococcus aureus , and Pseudomonas aeruginosa were lyzed with the bacteriolytic enzyme–collagen membrane. Relative activity of the bacteriolytic enzyme–collagen membrane against Pseu. solanacearum was about 2% of that of native bacteriolytic enzymes. No difference in the optimum pH was observed between immobilized enzymes and native enzymes. The bacteriolytic enzymes in the collagen membrane were stable in the pH range from 4 to 11. The enzyme–collagen membrane was stable against sodium chloride which was an inhibitor of the native bacteriolytic enzymes. Xanthomonas oryzae and Pseu. aeruginosa were continuously lyzed by a reactor containing the rolled bacteriolytic enzyme–collagen membrane.