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The use of α‐galactosidase and invertase in hollow fiber reactors
Author(s) -
Korus Roger A.,
Olson Alfred C.
Publication year - 1977
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260190102
Subject(s) - raffinose , invertase , chemistry , hydrolysis , enzyme , substrate (aquarium) , chromatography , levansucrase , bovine serum albumin , fiber , sucrose , immobilized enzyme , stachyose , biochemistry , organic chemistry , biology , ecology , genetics , bacillus subtilis , bacteria
Invertase and α‐galactosidase have been immobilized in hollow fiber cartridges with no detectable enzyme leakage and used for the hydrolysis of sucrose and raffinose, respectively. For both hollow fiber immobilizes enzymes nearly complete substrate conversion is possible. Enzyme stabilities in polysulfone hollow fibers which have been preconditioned with bovine albumin approach the stabilities of the free enzymes.