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A method for increasing cellulase production by Trichoderma viride
Author(s) -
Sternberg David
Publication year - 1976
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260181209
Subject(s) - trichoderma viride , cellulase , cellulose , hydrolysis , chemistry , food science , cellulosic ethanol , biochemistry
A doubling of cellulase production by Trichoderma viride (QM9414) is possible by increasing the cellulose concentration in the medium from 0.75 to 2%, increasing the nitrogen concentration, and controlling pH during growth. A four‐to fivefold increase in β ‐glucosidase is found with the higher cellulose concentration. Culture filtrates from 2% cellulose cultures can reduce the hydrolysis time in a practical saccharification to one‐half that required by culture filtrates from 0.75% cellulose cultures.