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Immobilized enzymes on chitosan columns: α‐Chymotrypsin and acid phosphatase
Author(s) -
Muzzarelli Riccardo A. A.,
Barontini Gaetano,
Rocchetti Roberto
Publication year - 1976
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260181011
Subject(s) - chitosan , enzyme , chitin , immobilized enzyme , chemistry , reagent , chymotrypsin , metal ions in aqueous solution , acid phosphatase , chromatography , biochemistry , metal , organic chemistry , trypsin
α‐Chymotrypsin and acid phosphatase have been immobilized on chitosan, a polyaminosaccharide, without using any intermediate reagent; the immobilized enzymes are active and their activity is much higher than for chitin‐immobilized enzymes. The best pH conditions for operating chitosan columns have been determined and columns have been used to transform substrates in large amounts, with no decrease of activity or enzyme losses. Due to the nonconvalent interaction between chitosan and enzymes, the pure and active enzymes can be eventually recovered from the columns. The effects of metal ions, aldehydes, and salts are reported and discussed. Applications are foreseen in the food and biomedical sciences and industries.