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Preparation and properties of bacterial chromatophores entrapped in polyacrylamide
Author(s) -
Yang Ho Seung,
Leung KwanHang,
Archer Michael C.
Publication year - 1976
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260181009
Subject(s) - polyacrylamide , rhodospirillum rubrum , photophosphorylation , chromatophore , chemistry , chromatography , biophysics , chemical engineering , biology , biochemistry , polymer chemistry , chloroplast , fishery , gene , enzyme , engineering
The immobilization of Rhodospirillum rubrum chromatophores was successfully performed by entrapping them in polyacrylamide. Their photophosphorylating activity was about 40% of native chromatophores. The temperature and pH optima for immobilized chromatophores were similar to the native photosynthetic apparatus and kinetic parameters showed that the rate of photophosphorylation in polyacrylamide particles was diffusion controlled. Light penetration of the gel particles was not a limiting parameter. Immobilization considerably increased the stability of the chromatophores towards denaturation.