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Invertase biosynthesis by Saccharomyces carlsbergensis in batch and continuous cultures
Author(s) -
Toda K.
Publication year - 1976
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260180807
Subject(s) - chemostat , invertase , dilution , saccharomyces , biosynthesis , enzyme , strain (injury) , biochemistry , biomass (ecology) , chemistry , biology , mutant , food science , saccharomyces cerevisiae , yeast , ecology , bacteria , genetics , thermodynamics , physics , anatomy , gene
The biosynthesis of invertase by Saccharomyces carlsbergensis LAM 1068 was studied in relation to its glucose effect at both unsteady and steady states of growth. Experimental correlations between the dilution rate and invertase specific activity ( E / X ) in chemostat, cultures led to an optimum for the enzyme synthesis at a particular intermediate growth rate. The value of E / X increased from 1.1 (U/mg biomass) in batch cultures to 13 (U/mg biomass) in chemostat cultures. A mutant strain A3 showed the highest value for E / X = 25 (U/mg biomass) at high dilution rates where glucose repression was observed with the wild strain.