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Pilot scale exponential growth of Escherichia coli W to high cell concentration with temperature variation
Author(s) -
Bauer S.,
White M. D.
Publication year - 1976
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260180606
Subject(s) - exponential growth , oxygen , growth rate , escherichia coli , fermentation , yield (engineering) , nutrient , ammonia , chemistry , volume (thermodynamics) , phosphorus , nitrogen , dry weight , constant (computer programming) , biology , zoology , food science , biochemistry , materials science , thermodynamics , botany , mathematics , mathematical analysis , geometry , physics , organic chemistry , computer science , metallurgy , gene , programming language
Abstract An efficient method to grow Escherichia coli W to high cell concentrations on the pilot scale is described and discussed. The method involves growth linked introduction of glucose; and ammonia to the culture, sparing with oxygen, and maintenance of aerobic conditions by gradually decreasing the temperature in the culture in order to keep the oxygen demand within the limits of the capacity of supply. Under these conditions the linear rate of cell mass production is actually the result of exponential growth with a gradually decreasing growth‐rate constant. About 10 kg packed cells were produced in a 50 liter working‐volume fermentor in one run of 13 hr. The concentration of the cells at the end of the growth was about 47 g dry cells/liter. The expenditure for nutrients was minimal and the controls were of simple automatic nature. From the determined yield constants for glucose, nitrogen, phosphorus, and oxygen it may be inferred that the cells grown by this method are similar to those grown exponentially at constant temperature.