Premium
A model for enzymatic isomerization of D ‐glucose to D ‐fructose in a batch reactor
Author(s) -
Sproull Robert D.,
Lim Henry C.,
Schneider Daniel R.
Publication year - 1976
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260180504
Subject(s) - fructose , chemistry , isomerization , glucose 6 phosphate isomerase , enzyme , catalysis , isomerase , kinetics , batch reactor , reaction rate constant , enzyme catalysis , equilibrium constant , biochemistry , thermodynamics , chromatography , physics , quantum mechanics
Using whole cells containing glucose isomerase, mathematical models for the enzymatic conversion of D ‐glucose to D ‐fructose and for the inactivation of the enzyme catalyst have been postulated and verified experimentally. The heat of reaction, the equilibrium constant, and the individual rate constants and their activation energies have been estimated. The model can be used to predict the time course for the enzymatic production of fructose in a batch reactor within the tested experimental range of 40–80°C.