Premium
Pilot plant production of glucose with glucoamylase immobilized to porous silica
Author(s) -
Lee Douglas D.,
Lee Yoon Y.,
Reilly Peter J.,
Collins Edgar V.,
Tsao George T.
Publication year - 1976
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260180210
Subject(s) - dextrin , hydrolysis , chemistry , immobilized enzyme , starch , chromatography , yield (engineering) , diffusion , enzyme , kinetics , porosity , amylase , chemical engineering , biochemistry , organic chemistry , materials science , physics , quantum mechanics , metallurgy , thermodynamics , engineering
Glucoamylase was immobilized to porous silica and its kinetics and stability were observed with acid‐ and α‐amylase‐hydrolyzed dextrin as feed. The enzyme was found to be extremely stable in both laboratory and pilot plant operations. When the feed had been previously only lightly hydrolyzed, pore diffusion limitation caused appreciable decreases in glucose production rate. The severity of starch hydrolysis to dextrin markedly affected ultimate glucose yields. The diffusional gradients present in the carrier pores caused the immobilized enzyme to yield lower glucose concentrations than the free enzyme at similar feed conditions.