Premium
Transient response of Enterobacter aerogenes under a dual nutrient limitation in a chemostat
Author(s) -
Cooney Charles L.,
Wang Daniel I. C.
Publication year - 1976
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260180205
Subject(s) - chemostat , nutrient , limiting , phosphate , biology , ammonia , microbiology and biotechnology , nitrogen , biochemistry , chemistry , bacteria , ecology , genetics , mechanical engineering , organic chemistry , engineering
Utilizing a chemostat with a dual nutrient limitation of nitrogen and phosphate, we examined the transient response of the culture following a pulse of one of the limiting nutrients (ammonia). This method provided quantitative evidence that cells can be grown under dual nutrient limitation. Furthermore, the pattern of response was consistent with the hypothesis that phosphate limitation restricts nucleic acid synthesis in the cell and that nitrogen limitation restricts protein synthesis. The net result is that under a phosphate limitation there is a restricted biosynthetic capacity which we feel is closely associated with the RNA content of the cell.