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Hydrolysis of D ‐galactosides in an open tubular lactase reactor
Author(s) -
Ngo T. T.,
Narinesingh D.,
Laidler K. J.
Publication year - 1976
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260180110
Subject(s) - lactose , lactase , chemistry , hydrolysis , skimmed milk , galactosides , substrate (aquarium) , diffusion , chromatography , galactose , biochemistry , food science , biology , thermodynamics , ecology , physics
Lactose (β‐galactosidase) was attached to the inner surface of nylon tubing. Tubes of various lengths were used to bring about the hydrolysis of o ‐nitrophenyl‐β‐ D ‐galactoside and of lactose in skim milk. The results with the former substrate were analyzed in the light of a theoretical treatment of Kobayashi and Laidler ( Biotechnol. Bioeng. , 16 , 99, 1974), with the conclusion that the reaction is intermediate between diffusion‐free and completely diffusion‐controlled behavior. The results with skim milk show that with a single 46 m tube and continuous circulation, 90% of the lactose is removed within 20 hr. A battery of ten such tubes, with single passage, at a flow rate of 2 cm/sec, would remove more than 99% of the lactose in less than 40 min.