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Engineering analysis of continuous production of L ‐aspartic acid by immobilized Escherichia coli cells in fixed beds
Author(s) -
Sato Tadashi,
Mori Takao,
Tosa Tetsuya,
Chibata Ichiro,
Furui Masakatsu,
Yamashita Kiyokazu,
Sumi Akihiko
Publication year - 1975
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260171209
Subject(s) - arrhenius plot , activation energy , substrate (aquarium) , arrhenius equation , chemistry , reaction rate constant , chromatography , escherichia coli , kinetics , aspartic acid , analytical chemistry (journal) , biochemistry , biology , amino acid , ecology , physics , quantum mechanics , gene
The reaction mechanism and decay behavior of aspartase activity for immobilized Escherichia coli cells were investigated by using a sectional packed column. Reaction within the immobilized cell column proceeded at zero‐order on substrate solutions ranging in concentration from 0.1 to 1.0 M , and the initial reaction rate was found to be 1.556 × 10 −2 mol/min/liter of immobilized cells. The effect of temperature on the reaction rate constant was investigated. The Arrhenius plot was straight line at temperatures below 43°C, and the activation energy for immobilized cells was calculated to be 12.36 kcal/mol. Asparatase activity in the immobilized cell column decayed exponentially and uniformly in all sections of a column. Its half‐life was approximately 120 days. The rate of formation of L ‐aspartic acid was shown to be independent of column dimensions.