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Some properties of a protease (subtilisin BPN′) immobilized to porous glass
Author(s) -
Chapman J. Douglas,
Hultin Herbert O.
Publication year - 1975
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260171208
Subject(s) - subtilisin , protease , porosity , chemistry , porous glass , chromatography , chemical engineering , materials science , enzyme , biochemistry , organic chemistry , engineering
Subtilisin BPN′ was immobilized to porous glass via isothiocyanate coupling. The pH optimum of the enzyme was shifted to the alkaline side on binding. This effect was more pronounced with ethyl lactate than with N ‐tosyl arginine methyl ester (TAME). Presumably, the shift is a reflection of the negative charge on the surface of the glass. The Michaelis constant and V max of soluble subtilisin BPN′ with TAME were two and one orders of magnitude, respectively, lower than with ethyl lactate. V max , calculated per g of active enzyme, with TAME as the substrate was not affected by immobilization, while V max with ethyl lactate decreased greater than tenfold. The apparent K M decreased on immobilization with ethyl lactate as substrate and increased with TAME. Results are explained in terms of diffusional resistance and a possible attraction of ethyl lactate to the glass surface. Active site titration indicated that about 25%, of the immobilized enzyme was active.