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Enzyme immobilized in a packed‐bed reactor: Kinetic parameters and mass transfer effects
Author(s) -
Marrazzo W. N.,
Merson R. L.,
McCoy B. J.
Publication year - 1975
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260171010
Subject(s) - mass transfer , kinetic energy , packed bed , chemistry , chromatography , enzyme , immobilized enzyme , chemical engineering , biochemistry , physics , engineering , quantum mechanics
To describe axial dispersion, particle film mass transfer, intraparticle diffusion, and the chemical reaction of the substrate for enzymes immobilized in porous particles in packed columns, we have developed mathematical models for first‐ and zero‐order limits of Michaelis‐Menten kinetics. Steady‐state solutions were derived for both long and short column boundary conditions and for plug flow. Theory was compared to experiments of hydrolysis of sucrose catalyzed by invertase bound to porous glass particles. Steady‐state conversions were measured for a range of flow rates. Pulse response experiments with inert packing were used to determine values of bed void fraction and particle porosity.