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Transient kinetics of yeast growth in batch and continous culture with an inhibitory carbon and energy source
Author(s) -
Ko R. Y. C.,
Edwards V. H.
Publication year - 1975
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260170703
Subject(s) - sodium acetate , exponential growth , kinetics , acetic acid , chemistry , zinc , steady state (chemistry) , dilution , chromatography , biochemistry , growth rate , biology , thermodynamics , organic chemistry , physics , mathematics , quantum mechanics , geometry
Abstract Growth and acetate metabolism by Candida utilis (ATCC 9226) is reported for both acetate‐ and zinc‐limited cultures in defined media. Acetate concentrations were varied from suboptimal to inhibitory levels in both types of media in differential shake flask culture and in batch and continuous cultures in stirred fermentors. Transient responses of steady‐state cultures to small or large additions of concentrated sodium acetate, or to shifts in dilution rate or inlet acetate concentration are compared with one another and with simple mathematical models of growth and acetate metabolism. Exponential growth was observed during unrestricted growth (differential shake flask and batch cultures) with both types of media. Addition of acetate during unrestricted growth always caused lags and for larger pulses, lower specific growth rates were observed after exponential growth resumed. Inhibition by high acetate concentrations was much greater in acetate–limited than in zinc–limited cultures. During restricted growth (steady‐state, continuous cultures), high acetate concentrations again consistently caused growth lags but stimulated, inhibited, or temporarily stopped acetage uptake. Qualitative agreement between the predictions of a simple mathematical model of acetate inhibition fitted to differential shake flask data and the observed transient data was surprisingly good.