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Kinetics of biosynthesis of polyene macrolide antibiotics in batch cultures: Cell maturation time
Author(s) -
Martin Juan F.,
McDaniel Lloyd E.
Publication year - 1975
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260170611
Subject(s) - polyene , chemistry , fermentation , mycelium , macrolide antibiotics , biochemistry , biosynthesis , extracellular , antibiotics , food science , biology , botany , erythromycin , enzyme
The changes in several parameters of the candicidin fermentation (total, mycelium‐associated, and extracellular product formation; growth rate; DNA content; glucose utilization; dissolved oxygen in the broth; and oxygen uptake rate) during the trophophase‐idiophase transition are compared with previously reported data for the polyene macrolides, candidin and candihexin. The maturation time, t m , and the productformation rate constant, k p , have been calculated for each of the three polyene macrolides using a simple mathematical model. Slow‐feeding of glucose, which resulted in candidin and candihexin overproduction has been shown to increase the polyene formation rate constant and toretard trophophase to idiophase transition (longer maturation time). The opposite effect is achieved by repeated feeding of soybean meal. The values of the maturation times and polyene formation rates obtained were used to predict the production of polyene macrolide antibiotics in batch cultures.