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Maintenance of perpetual synchrony in HeLa suspension cultures: Question of unbalanced growth
Author(s) -
Thilly W. G.,
Arkin D. I.,
Nowak T. S.,
Wogan G. N.
Publication year - 1975
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260170507
Subject(s) - hela , suspension culture , dna synthesis , cell division , dna , suspension (topology) , cell growth , growth inhibition , division (mathematics) , microbiology and biotechnology , biology , cell culture , biophysics , cell , chemistry , biochemistry , genetics , mathematics , arithmetic , homotopy , pure mathematics
Unbalanced growth has been studied in HeLa cell cultures maintained in perpetual division synchrony by periodic inhibition of DNA synthesis. Observation of eight independent cellular parameters indicates that once synchrony has been established the degree of unbalanced growth in later cycles is not as great as is observed with the conventional “double‐block” technique. This significant diminution of unbalanced growth appears to be related to the significantly shorter periods of inhibition of DNA synthesis used in the resynchronization process.