Temporal structure of S phase analyzed using an automated synchrony system | Zendy

Klevecz Robert R. | Zendy

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Temporal structure of S phase analyzed using an automated synchrony system

Author(s) -

Klevecz Robert R.

Publication year - 1975

Publication title -

biotechnology and bioengineering

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.136

H-Index - 189

eISSN - 1097-0290

pISSN - 0006-3592

DOI - 10.1002/bit.260170505

Subject(s) - mitosis , synchronization (alternating current) , biological system , chinese hamster , cell cycle , biology , microbiology and biotechnology , dna synthesis , phase (matter) , point (geometry) , cell , cell division , cell culture , dna , computational biology , computer science , chemistry , genetics , mathematics , channel (broadcasting) , geometry , organic chemistry , computer network

An automated system for cell cycle analysis is described in which synchronous populations of greater than 95% mitotic cells can be selected and subcultured without resorting to inhibitors or altered media. Selection of mitotic cells from roller bottle cultures generates synchronous populations of up to 5 × 10 7 cells, as often as every 30 min, under conditions of constant temperature. A detailed analysis of the temporal pattern of DNA synthesis in the S phase of several Chinese hamster cells is presented. Several potential artefacts and uncertainties regarding the cell cycle blockage point in S phase synchronization are discussed.

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