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Conversion of L ‐sorbose to L ‐sorbosone by Gluconobacter melanogenus
Author(s) -
Kitamura I.,
Perlman D.
Publication year - 1975
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260170305
Subject(s) - sorbose , thin layer chromatography , chromatography , chemistry , betaine , incubation , column chromatography , choline , biochemistry , fructose
Growing cultures, washed cells, and cell‐free extracts of Gluconobacter melanogenus IFO 3293 were found to convert L ‐sorbose to L ‐sorbosone. The product was identified by thin layer chromatography of the 2, 4‐dinitrophenylhydrazone, and by paper partition chromatography using chemically prepared materials as standards. Factors influencing the conversion included incubation temperature and composition of the growth medium. Addition of betaine or choline to the growing cultures stimulated conversion of L ‐sorbose to L ‐sorbosone.