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Enzyme immobilization on macroreticular polystyrene
Author(s) -
Baum George
Publication year - 1975
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260170210
Subject(s) - papain , polystyrene , immobilized enzyme , enzyme , chemistry , covalent bond , covalent binding , biochemistry , chromatography , organic chemistry , polymer
Abstract Macroreticular polystyrene beads may be converted into suitable supports for covalent binding of enzymes. In many respects the supports are physically similar to controlled pore glass (CPG). Our results for immobilized glucoamylase were very similar to published results using CPG as a carrier. The characteristics of immobilized papain were less satisfactory. The product exhibited a Z‐shaped activity‐time profile suggestive of the involvement of multiple mechanisms.