Premium
High‐yield growth of E. coli at different temperatures in a bench scale fermentor
Author(s) -
Shiloach J.,
Bauer S.
Publication year - 1975
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260170208
Subject(s) - yield (engineering) , wax , fermentation , nitrogen , chemistry , oxygen , ammonia , growth rate , phosphorus , exponential growth , chromatography , food science , materials science , biochemistry , organic chemistry , geometry , mathematics , metallurgy , mathematical analysis
E. coli wax grown exponentially at different temperatures in a bench scale fermentor. pH was maintained at 6.8 by ammonia which served also as the nitrogen source. Glucose was introduced semi‐continuously at a predetermined rate which ensured a glucose concentration of 25–50 g/liter during growth. The culture was sparged with pure oxygen. Yield constants for glucose, nitrogen, phosphorus, and oxygen were determined at the different temperatures of propagation. When all growth conditions, except temperature, were kept constant, the maximal possible yield of exponentially grown cell mass was found to be directly proportional to the doubling time. Concentrations of up to 55 g dry cells/liter culture were achieved.