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Starch conversion by soluble and immobilized α‐amylase
Author(s) -
Linko YuYen,
Saarinen P.,
Linko M.
Publication year - 1975
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260170203
Subject(s) - amylase , maltose , chemistry , starch , immobilized enzyme , hydrolysis , chromatography , enzyme , biochemistry
B. subtilis α‐amylase was immobilized on cyanogen bromide activated carboxymethyl cellulose. The conversion of wheat starchwas carried out at 72°C in a stirred tank by soluble and immobilized α‐amylase. The initial reaction rate with immobilized α‐amylase was lower than with the soluble enzyme, but after 1 hr immobilized α‐amylase produced a higher quantity of reducing sugars than the soluble enzyme. The action pattern of immobilized α‐amylase was different from that of the soluble enzyme: immobilized α‐amylase produced relatively more glucose and maltose, except at the beginning of conversion. Immobilized α‐ readily hydrolyze G 6 . The starch conversion by immobilized α‐amylase was not diffusion controlled at a stirring rate of 100‐300 rpm.