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Application of an insolubilized hepatic microsomal drug oxidase to the continous production of N ‐oxides
Author(s) -
Sofer Samir S.,
Ziegler Daniel M.,
Popovich Robert P.
Publication year - 1975
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260170109
Subject(s) - catalysis , chemistry , ethylmorphine , microsome , yield (engineering) , immobilized enzyme , chromatography , nuclear chemistry , enzyme , organic chemistry , materials science , metallurgy
The optimum operating temperature and pH have been determined for a catalyst prepared by the insolubilization of a hepatic microsomal mixed‐function oxidase on glass beads. Temperature is a more significant variable than pH in determining potential catalyst yield (total product/unit catalyst). An operating range of about 25–28°C is optimal with respect to catalyst life at pH 7.6. Like the purified soluble oxidase, the glass bead‐mounted enzyme catalyzes the NADPH‐ and oxygen‐dependent oxidation of a variety of amines, hydrazines, and thioureylenes. The catalyst has been used in a simple slurry reactor to prepare mg quantities of N ‐oxide metabolites of prochlorperazine, guanethidine, and ethylmorphine.