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Cofactor recycling in an enzyme reactor. A comparison Using free and immobilized dehydrogenases with free and immobilized NAD
Author(s) -
Wykes J. R.,
Dunnill P.,
Lilly M. D.
Publication year - 1975
Publication title -
biotechnology and bioengineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.136
H-Index - 189
eISSN - 1097-0290
pISSN - 0006-3592
DOI - 10.1002/bit.260170105
Subject(s) - nad+ kinase , cofactor , chemistry , enzyme , alcohol dehydrogenase , biochemistry , alcohol , derivative (finance) , financial economics , economics
The requirements for enzymic cofactor recycling have been investigated in a system employing alcohol and lactate dehydrogenases. The interactions of various combinations of free dehydrogenases or dehydrogenases immobilized either to the same or separate supports, with free NAD, a soluble highmolecular weight derivative of NAD or an insoluble derivative of NAD have been examined.